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ACT Natural Micro-fiber Cloth and Mop Microbiological Use Study December 1998
I. Background
Euronet USA contracted a world-recognized independent testing laboratory (found on the Internet at www.actstesting.com) to test the ACTEX® Multi-purpose Cloth and the ACTEX® Damp-mop for cleaning effectiveness. The information in this fact sheet consists of summarized results, compiled numerical data, and direct quotes all resulting from the technical report provided by the microbiology laboratory.
The purpose of this study was "to develop and perform a test method which would demonstrate the efficacy of the ACT Natural Cloths and Mops in removing microbiological contaminants from various surface types."
The question has never been do these products clean the dirt that we can see - all one needs to do is try them to be convinced that they work. But the question has been, how well do they clean the dirt (contamination) that we do not see. Therefore, throughout this testing two different microorganisms (Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739) were used along with normal household dirt particles taken from a vacuum bag. In addition to testing the efficacy of the cloths/mops to clean hard (non-porous) surfaces, the laboratory also conducted tests on cloth saturation, cross-contamination, and methods used to clean dirty (contaminated) cloths. All tests were conducted in triplicate and data depicted in this fact sheet reflects the average of all three tests.
II. Executive Summary
The Executive Summary from the technical laboratory report provides a summation of several key areas from the testing and is provided in its entirety below:
"The ACT Natural Cloths removed an average of 99.94% of all microorganisms throughout the test period. Only the Conventional Cloth in conjunction with a disinfectant (which had to remain on the test surface for 30 seconds) performed better. Using the ACT Natural cloth with water provides clean surfaces without the use of harsh chemicals. The effectiveness of these cloths was not reduced even after cleaning 10 surfaces in succession as demonstrated by the absorption test data.
The ACT Natural Mops were significantly more effective in removing bacteria than the Conventional Mops. In the first series of tests with the mops, the conventional mops were "new", they had not been used and were dry. After use, we boiled the mops, rung them out, then left them at room temperature. These mops developed a significant bioburden (bacterial population) under these "normal use" conditions. This is demonstrated by the second set of test data (for the ceramic surface) where there were more bacteria on the test surface after cleaning than before. Therefore, the repeat use of a conventional mop, even with a disinfectant, actually adds bacteria to the surface being cleaned."
The testing also demonstrated that after a contaminated (dirty) area had been cleaned with the ACTEX® Multi-purpose Cloth, the contamination was held inside the fiber of the cloth and was not transferred to another surface. Lastly, laboratory testing showed that washing the cloths in a standard washing machine on the hottest cycle was as effective in removing bacteria from the cloth as was boiling. Specific details of each test are provided below.
III. Individual Test Results
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ACTEX® Multi-purpose Cloth Testing. Testing was conducted using two types of Formica countertop material (one very smooth and one with a slight texture - both surfaces are typical of those found in a kitchen.) All surfaces were contaminated with a solution containing two microorganisms (Staphylococcus aureus and Escherichia coli) and common dirt taken from a vacuum cleaner bag. After the solution dried, surfaces were cleaned with the ACTEX® Multi-purpose Cloth (new and used) using only water and a conventional cloth with three different cleaning solutions - Lysol® Pine Action Cleaner, Formula 409®, and Tops Regular Scent Bleach. Lysol® was used as a cleaner in accordance with the directions on the container ("29.5 mLs of cleaner mixed with 473mLs of water"). Formula 409® was used as a disinfectant in accordance with the directions on the container (spray surface with full strength 409® until thoroughly wet, let stand 30 seconds, then wipe). Bleach was used as a disinfectant in accordance with health department guidelines. (Note: Arizona Health Department and Arizona law stipulate that bleach water with a concentration of bleach between 50 parts-per-million (ppm) and 100 ppm is required for disinfecting surfaces in restaurants, etc. This has become a recognized "standard" for cleaning effectiveness.) The ACTEX® Multi-purpose Cloths were moistened with water until their weight had doubled. After the surfaces were cleaned with each method above, the surfaces were compared with control surfaces that were not cleaned. The comparison of remaining contamination on the cleaned surface to the contamination on the control surface results in an effectiveness rating expressed as a percentage (i.e. "99.94% effective" reflects the result expressed in the Executive Summary above).
(1) Compiled results on the ACTEX® Multi-purpose Cloth:
Table 1: Cloth Testing Smooth Formica Surface
| Type of Cleaning Material |
Cleaning Agent |
Percent Effective (%) |
| ACT-tex® Multi-purpose Cloth (New) * |
Tap Water |
99.9511 % |
| ACT-tex® Multi-purpose Cloth (Used) * |
Tap Water |
99.9506 % |
| Conventional Dish Cloth (New) |
Lysol® Pine Action Cleaner |
92.7778 % |
| Conventional Dish Cloth (New) |
409® as Disinfectant |
99.9994 % |
| Conventional Dish Cloth (New) |
Bleach Water (60 ppm) |
99.0000 % |
* "New" is defined as 12 or less previous uses, and "used" is defined as more than 50 uses.
Table 2: Cloth Testing Textured Formica Surface
| Type of Cleaning Material |
Cleaning Agent |
Percent Effective (%) |
| ACT-tex® Multi-purpose Cloth (New) * |
Tap Water |
99.9721 % |
| ACT-tex® Multi-purpose Cloth (Used) * |
Tap Water |
99.9646 % |
| Conventional Dish Cloth (New) |
Lysol® Pine Action Cleaner |
90.3571 % |
| Conventional Dish Cloth (New) |
409® as Disinfectant |
99.9977 % |
| Conventional Dish Cloth (New) |
Bleach Water (60 ppm) |
98.7073 % |
* "New" is defined as 12 or less previous uses, and "used" is defined as more than 50 uses.
(2) Analysis of Results:
These results demonstrate that the ACTEX® Multi-purpose Cloth is extremely effective. Only Formula 409® used as a disinfectant did better - remember that the surface was saturated with a full concentration of 409® and allowed to stand for 30 seconds. Of particular note is the comparison of the ACTEX® Multi-purpose Cloth with the bleach water solution which is recognized as a standard for cleanliness. Even though the percentages are very close, a mathematical analysis of these percentages shows that the bleach water left much more contamination on the surfaces as compared to the ACTEX® Multi-purpose Cloth (20 times the amount of contamination on the smooth surface and 46 times on the textured surface).
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ACTEX® Damp-mop Testing. Testing was conducted on two types of flooring (vinyl composition floor tile and ceramic floor tile - both surfaces are typical of those found in a home environment.) All surfaces were contaminated with a solution containing two microorganisms (Staphylococcus aureus and Escherichia coli) and common dirt taken from a vacuum cleaner bag. After the solution dried, surfaces were cleaned with ACTEX® Damp-mop (new and used) using only water and a conventional cotton mop with Lysol® Pine Action Cleaner -- used both as a cleaner and as a disinfectant. Lysol® was used as a cleaner in accordance with the directions on the container ("29.5 mLs of cleaner mixed with 473mLs of water") and "was used to wipe the surfaces without letting the product stand on the surfaces". Using the same dilution, Lysol® "was used as a disinfectant for the floor surfaces by allowing the product to remain "wet" on the surface for 10 minutes as per bottle instructions". The ACTEX® Damp-mops were moistened with water to 175% of their dry weight. After the surfaces were cleaned with each method, the surfaces were compared with control surfaces that were not cleaned. The comparison of remaining contamination on the cleaned surface to the contamination on the control surface results in an effectiveness rating expressed as a percentage. It is important to remember, when reviewing the results of this test that "the conventional mops were "new", they had not been used and were dry (see Table 3 below). After use, we boiled the conventional (added) mops, rung them out, then left them at room temperature (Table 4 below)."
(1) Compiled results on the ACTEX® Damp-mop:
Table 3: Mop Testing Floor Tile-Vinyl Composition
| Type of Cleaning Material |
Cleaning Agent |
Percent Effective (%) |
| ACT-tex® Damp Mop (New) * |
Tap Water |
99.3710 % |
| ACT-tex® Damp Mop (Used) * |
Tap Water |
98.8710 % |
| Conventional Cotton Mop (New) |
Lysol® Pine Action Cleaner |
84.0323 % |
| Conventional Cotton Mop (New) |
Lysol® as Disinfectant |
99.7419 % |
* "New" is defined as 12 or less previous uses, and "used" is defined as more than 50 uses.
Table 4: Mop Testing Floor Tile-Ceramic
| Type of Cleaning Material |
Cleaning Agent |
Percent Effective (%) |
| ACT-tex® Damp Mop (New) * |
Tap Water |
91.1765 % |
| ACT-tex® Damp Mop (Used) * |
Tap Water |
94.1176 % |
| Conventional Cotton Mop (New) |
Lysol® Pine Action Cleaner |
39.7059 % |
| Conventional Cotton Mop (New) |
Lysol® as Disinfectant |
0.0000 % |
* "New" is defined as 12 or less previous uses, and "used" is defined as more than 50 uses.
(2) Analysis of Results:
These results demonstrate that the ACTEX® Damp-mop is more effective than a conventional mop. The most significant aspect of the mop testing is what happens to the conventional mop after being used for the first time. The conventional mops used on the ceramic tile surface had been used only once on the previous test. These mops were "0%" effective on the second test even using a disinfectant. A conventional mop may make the floor surface "look" cleaner; however, the "cleaned" surface retains very high levels of bacterial contamination (microorganisms).
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ACTEX® Multi-purpose Cloth Saturation Testing. During this test, ten (10) identical smooth Formica surfaces were cleaned with the same ACTEX® Multi-purpose Cloth using only water (as in cloth testing above). "This means that the cloths were folded in four and that the same cloth surface was used to wipe each of the ten test surfaces in succession". After all surfaces were cleaned, the surfaces were compared with control surfaces that were not cleaned. The comparison of remaining contamination on the cleaned surface to the contamination on the control surface results in an effectiveness rating expressed as a percentage.
(1) Compiled results on the ACTEX® Multi-purpose Cloth Saturation Testing:
Table 5: ACT-tex® Multi-purpose Cloth Saturation Testing
| Surface Number |
Percent Effective (%) |
| Surface 1 |
99.9467 % |
| Surface 2 |
99.8533 % |
| Surface 3 |
99.8800 % |
| Surface 4 |
99.9847 % |
| Surface 5 |
99.5000 % |
| Surface 6 |
99.4667 % |
| Surface 7 |
99.7267 % |
| Surface 8 |
99.2667 % |
| Surface 9 |
99.8900 % |
| Surface 10 |
99.7633 % |
| Average |
99.7278 % |
(2) Analysis of Results:
The test demonstrates that the ACTEX® Multi-purpose Cloth can clean a large surface before the fibers becoming saturated with microorganisms. Based on the size of the surfaces cleaned and the size of the cloth folded in quarters, a single ACTEX® Multi-purpose Cloth could clean up to 40 square feet of Formica counter-top with over a 99% effectiveness rate. Remember, the "recognized standard for cleanliness" using bleach water was between 98.7% and 99.0%. Further testing would be required to identify the maximum area that could be "cleaned" by a single cloth (e.g. how much area could be cleaned before the cleaning effectiveness of the cloth fell below the "standard").
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ACTEX® Multi-purpose Cloth Cross-Contamination Test. For this test, ACTEX® Multi-purpose Cloths that had been used to clean contaminated surfaces were then used to wipe "clean" surfaces. "These "clean" surfaces, along with three control (not wiped) surfaces were then swabbed to see how many bacteria were present." The comparison of contamination left by the ACTEX® Multi-purpose Cloth on the "clean" surfaces to the contamination on the control surfaces results in a relative level of contamination or cleanliness both expressed as a percentage.
(1) Compiled results on the ACTEX® Multi-purpose Cloth Cross-Contamination Testing:
Table 6: ACT-tex® Multi-purpose Cloth Cross-Contamination Test
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% Contamination |
% Clean |
| Control Surface Cleanliness |
0.0006 % |
99.9995 % |
| ACT Natural Cloth on Clean Surface |
0.0279 % |
99.9721 % |
(2) Analysis of Results:
This Test demonstrates that the ACTEX® Multi-purpose Cloth retains the contamination from a cleaned surface within it's fibers and this contamination is not transferred to another surface (subject to saturation limits of the cloth).
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ACTEX® Multi-purpose Cloth -- Washing vs. Boiling Test. For this test, ACTEX® Multi-purpose Cloths that had been used to clean like contaminated surfaces (i.e. the relative level of contamination on the dirty cloths was the same) were cleaned using two different methods. One set of cloths was washed in a Kenmore Series 70 Extra Capacity Washer (normal cycle of 40 minutes with a 12-minute wash cycle at a temperature of 120°F) using a mild detergent (Tide®). The other set of cloths was boiled (as recommended, three-five minutes). After cleaning, both sets of cloths were tested for contamination. The comparison of the contamination left in the ACTEX® Multi-purpose Cloth to the level of contamination cleaned from the contaminated surface results in a relative level of contamination or cleanliness both expressed as a percentage.
(1) Compiled results on the ACTEX® Multi-purpose Cloth - Washing vs. Boiling Testing:
Table 7: ACT-tex® Multi-purpose Cloth - Washing vs. Boiling Testing
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% Contamination |
% Clean |
| ACT Natural Cloth - Boiled |
0.000036 % |
99.999964 % |
| ACT Natural Cloth - Machine Washed |
0.000029 % |
99.999971 % |
(2) Analysis of Results:
These results demonstrate that washing the cloths in a washing machine on a normal cycle with a mild detergent and relatively hot water (120°F) cleans the cloth as effectively as boiling (effectiveness measured in ability to remove bacteria/microorganisms from the cloth and not in removing stains).
IV. Conclusion
The ACTEX® Multi-purpose Cloth and the ACTEX® Damp-mop both clean hard (non-porous) surfaces in a highly effective manner without the use of harsh household chemicals. "Effectiveness" here is measured by the ability of these products to clean the dirt (contamination) that we do not see as well as cleaning the dirt and grime that we do see.
Questions and comments should be directed to Euronet USA, 4811 E. Julep Street, Suite 120, Mesa, Arizona 85215. Or write an Email now.
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